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Updated: Jun 12, 2026

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Biotransformation of ent-kaur-16-en-19-oic acid by Psilocybe cubensis
Jaraslak Pechwang1, Prakitsin Sihanonth, Surachai Pornpakakul
1Biotechnology Programme, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Abstract:
Biotranformation of ent-kaur-16-en-19-oic acid (1) using Psilocybe cubensis resulted in hydroxylated products. After two days of incubation, ent-16beta,17-dihydroxy-kauran-19-oic acid (2) was isolated. After further incubation for nine days, two novel metabolites, ent-12alpha,16beta,17-trihydroxy-kauran-19-oic acid (3) and ent-11alpha,16beta,17-trihydroxy-kauran-19-oic acid (4), were obtained. The metabolites were identified by spectroscopic methods and X-ray crystallography. Compounds 1-4 were evaluated for their cytotoxic properties against the human leukaemia K562 cell line; only compound 1 showed moderate activity.
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