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Updated: Jun 12, 2026
06:57
Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019
In situ hybridization for human papillomavirus
Olga Stasikowska-Kanicka1, Marian Danilewicz, Małgorzata Wagrowska-Danilewicz
1Olga Stasikowska-Kanicka MSc, PhD, Department of Nephropathology Medical University of Lodz, ul. Czechosłowacka 8/10, 92-216 Łódź, phone/fax +48 42 679 01 91,
Summary
Human papillomavirus (HPV) plays a role in laryngeal cancers and sinonasal inverted papillomas. However, the study found no direct link between HPV DNA presence and visible HPV infection signs in these head and neck lesions.
Area of Science:
- Oncology
- Virology
- Pathology
Background:
- Growing evidence links human papillomavirus (HPV) to specific head and neck cancers.
- HPV is implicated in the development of certain laryngeal squamous cell carcinomas and sinonasal inverted papillomas.
Purpose of the Study:
- To investigate the association between HPV DNA and morphological indicators of HPV infection.
- To evaluate HPV DNA presence in laryngeal squamous cell carcinomas and sinonasal inverted papillomas.
Main Methods:
- Analysis of 26 laryngeal squamous cell carcinomas and 17 sinonasal inverted papillomas using archival formalin-fixed, paraffin-embedded tissues.
- In situ hybridization was employed to detect HPV DNA expression.
- Morphological assessment for signs of HPV infection was performed.
Main Results:
- Morphological signs of HPV infection were observed in 10/26 cancers and 11/17 papillomas.
- HPV DNA was detected in both cancer and papilloma groups when morphological signs were present.
- No significant difference in HPV DNA frequency was found between cases with and without morphological signs of infection.
Conclusions:
- This study confirms the involvement of HPVs in the pathogenesis of inverted papillomas and laryngeal squamous cell carcinomas.
- A significant relationship between HPV DNA presence and morphological signs of HPV infection was not established in the studied cases.
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02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...