Related Experiment Video
Updated: Jun 12, 2026
08:01
The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
Published on: February 27, 2026
[Laboratory examinations in antiphospholipid syndrome]
Masahiro Ieko1, Sumiyoshi Naito, Mika Yoshida
1Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-gun, Hokkaido 061-0293, Japan. iekom@hoku-iryo-u.ac.jp
Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 26, 2010
Summary
This study refines lupus anticoagulant (LA) detection methods for antiphospholipid syndrome (APS) diagnosis. Optimized cross-mixing tests show promise for accurate LA identification, improving APS diagnostic capabilities.
Area of Science:
- Hematology
- Immunology
- Clinical Diagnostics
Context:
- Antiphospholipid syndrome (APS) diagnosis relies on detecting antiphospholipid antibodies (aPL).
- Current aPL detection methods face challenges, including issues with IgM-class antibody measurements and standardization.
- Lupus anticoagulant (LA) is a key marker for APS-related thrombosis.
Purpose:
- To evaluate optimized cross-mixing tests for lupus anticoagulant (LA) detection.
- To address limitations in current LA detection methodologies.
- To improve the accuracy and reliability of aPL testing for APS diagnosis.
Summary:
- The study investigated LA detection using activated partial thromboplastin time (APTT) reagents with varying patient plasma concentrations (0-100%).
- Favorable results were obtained for LA detection via cross-mixing tests with filtered patient plasma.
- This approach addresses limitations in current LA detection, particularly concerning reagent sensitivity and standardization.
Impact:
- Potential to enhance the accuracy of antiphospholipid syndrome diagnosis.
- May lead to improved laboratory testing protocols for aPL.
- Contributes to the evolving understanding and diagnostic criteria for APS.
Related Concept Videos
01:20Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies
The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
01:21Peripheral Arterial Disease II: Clinical Manifestations and Diagnostic Evaluation
Clinical manifestationsPeripheral Arterial Disease (PAD) manifests through a range of symptoms, from the characteristic intermittent claudication to atypical presentations and severe complications in advanced stages. Intermittent claudication, a hallmark symptom of PAD, presents as exercise-induced muscle pain that typically resolves within minutes of rest. This pain is reproducible and stems from inadequate blood flow, leading to the accumulation of lactic acid produced during anaerobic...
01:30Acute Coronary Syndrome III: Diagnostic Studies
Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
01:22Rheumatic Heart Disease II: Clinical Manifestations and Diagnostic Studies
The key clinical manifestations of Rheumatic heart disease (RHD) include several distinct cardiac symptoms.Carditis, a hallmark of acute rheumatic fever, involves inflammation of the heart's endocardium, myocardium, and pericardium. Chronic RHD often results from recurrent episodes of carditis. Its symptoms include the following:Murmurs are caused by valvular damage, especially to the mitral and aortic valves. Mitral stenosis or regurgitation is common, with characteristic heart murmurs...
01:29Pulmonary Embolism II: Diagnostic Studies and Interprofessional Care
Diagnosing Pulmonary EmbolismDiagnosing pulmonary embolism (PE) involves clinical assessment and advanced imaging tests. The preferred diagnostic tool is the spiral (helical) CT scan or CT angiography (CTA), which uses intravenous contrast media to visualize the pulmonary vasculature and identify emboli.A ventilation-perfusion (V/Q) scan is an alternative for patients unable to receive contrast media. This scan includes both perfusion and ventilation scanning. Perfusion scanning involves...
01:27Atherosclerosis II: Clinical Manifestations and Diagnostic Tests
Atherosclerosis is a progressive disorder that leads to the thickening and narrowing of arterial walls due to plaque buildup. This condition can cause various symptoms depending on the arteries affected:Coronary Artery Disease (CAD): This condition affects the coronary arteries and may lead to chest pain (angina), shortness of breath (dyspnea), heart attacks, and other heart disease symptoms.Cerebrovascular Disease: This affects blood flow to the brain, causing transient ischemic attacks (TIAs)...