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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

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Related Experiment Video

Updated: Jun 12, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Imaging with Raman spectroscopy.

Yin Zhang1, Hao Hong, Weibo Cai

  • 1Department Medical Physics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705-2275, USA.

Current Pharmaceutical Biotechnology
|May 26, 2010
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy offers detailed chemical analysis for biomedical applications like cancer diagnosis. Advanced techniques like CARS, SERS, and SWNTs enhance sensitivity for biological imaging, overcoming some limitations.

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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

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Last Updated: Jun 12, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Chemical Imaging

Background:

  • Raman spectroscopy, leveraging inelastic photon scattering, is a versatile analytical tool.
  • Its application is expanding into biomedical fields, particularly for cancer diagnosis, due to its ability to reveal cellular and tissue chemical composition.
  • Enhanced sensitivity is crucial for imaging applications, driving the development of specialized Raman techniques.

Purpose of the Study:

  • To review advancements in Raman spectroscopy-based imaging techniques.
  • To summarize key methods including Coherent Anti-Stokes Raman Spectroscopy (CARS), Surface-Enhanced Raman Spectroscopy (SERS), and Single-Walled Carbon Nanotubes (SWNTs).
  • To highlight the potential and limitations of these techniques for biomedical applications.

Main Methods:

  • Coherent Anti-Stokes Raman Spectroscopy (CARS) for lipid C-H bond imaging.
  • Surface-Enhanced Raman Spectroscopy (SERS) utilizing nanoparticles as contrast agents.
  • Single-Walled Carbon Nanotubes (SWNTs) leveraging intrinsic Raman signals.

Main Results:

  • Raman spectroscopy imaging has seen significant progress with successful proof-of-principle experiments.
  • SERS offers multiplexing capabilities for simultaneous interrogation of multiple biological events.
  • CARS is effective for imaging lipid-rich structures.

Conclusions:

  • Raman spectroscopy imaging holds immense potential for simultaneous multi-analyte detection in biological systems.
  • Limited tissue penetration remains a key challenge for in vivo human applications, similar to other optical methods.
  • Continued advancements are expected in Raman spectroscopy imaging over the next decade.