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Updated: Jun 12, 2026
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
Published on: August 27, 2021
Phage display applications for molecular imaging
Jiong Cai1, Zhaofei Liu, Fan Wang
1Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
Abstract:
The advancement of molecular imaging hardware and disease targets has driven the demand of novel probes for diagnosis of human diseases. The high-throughput property of phage-display techniques makes the fast developments of these probes possible. The antibody and peptide phage display libraries have been widely used in ligand-selections for a variety of targets in tumors including osteosarcomas, pancreatic ductal adenocarcinomas, thyroid, head and neck squamous cell carcinomas, prostate, bladder, colon and gastric cancers. The phage display techniques also have been utilized in development of imaging agents for tumor therapy response, apoptosis, hypoxia, infection and inflammation, Alzheimer's diseases, diabetes and cardiovascular diseases.
Insights
Phage display technology accelerates the development of molecular imaging probes for diagnosing various human diseases, including cancers and neurological conditions. This high-throughput method enables rapid identification of ligands for diverse disease targets.
Area of Science:
- Biomedical engineering
- Molecular imaging
- Drug discovery
Background:
- Advancements in molecular imaging hardware and disease targets necessitate novel diagnostic probes.
- Phage display techniques offer high-throughput capabilities for rapid probe development.
Purpose of the Study:
- To highlight the utility of phage display in developing molecular imaging probes.
- To showcase the application of phage display for various disease targets.
Main Methods:
- Utilizing antibody and peptide phage display libraries for ligand selection.
- Applying phage display techniques for the development of imaging agents.
Main Results:
- Phage display has been successfully applied to identify ligands for numerous cancer types, including osteosarcomas, pancreatic cancer, and thyroid cancer.
- The technique is also used for developing agents targeting tumor therapy response, apoptosis, hypoxia, infections, inflammation, Alzheimer's disease, diabetes, and cardiovascular diseases.
Conclusions:
- Phage display is a powerful and versatile tool for accelerating the development of molecular imaging probes.
- This technology has broad applications in diagnosing and monitoring a wide range of human diseases.
