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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 12, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

18F-labeled proteins.

Zhanhong Wu1, Fouad Kandeel

  • 1Department of Diabetes, Endocrinology & Metabolism, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA. zwu@coh.org

Current Pharmaceutical Biotechnology
|May 26, 2010
PubMed
Summary

Positron emission tomography (PET) imaging utilizes radiolabeled proteins for diagnostics and therapy monitoring. This review details methods for synthesizing novel fluorine-18 labeled proteins, crucial for advanced molecular imaging applications.

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Area of Science:

  • Molecular imaging
  • Radiochemistry
  • Biomedical science

Background:

  • Positron emission tomography (PET) is a key molecular imaging technique.
  • PET visualizes human physiology using positron-emitting radiopharmaceuticals.
  • It provides insights into metabolism, receptor/enzyme function, and biochemical mechanisms.

Purpose of the Study:

  • To review key aspects of protein labeling with fluorine-18 ((18)F).
  • To discuss strategies for synthesizing novel (18)F-labeled proteins.
  • To highlight the importance of (18)F-labeled proteins for diagnosis and therapy monitoring.

Main Methods:

  • Focuses on strategies for synthesizing (18)F-labeled proteins.
  • Emphasizes the use of (18)F-containing prosthetic groups for efficient labeling.
  • Discusses methods to maintain protein bioactivity under mild labeling conditions.

Main Results:

  • Highlights advancements in protein (18)F-labeling methodologies.
  • Presents representative examples of successful (18)F-labeled proteins.
  • Includes (18)F-labeled human serum albumin, Annexin V, HER2 affibody, and low-density lipoprotein.

Conclusions:

  • Efficient synthesis of (18)F-labeled proteins is critical for PET imaging.
  • Prosthetic group strategies enable high-yield, mild labeling crucial for protein integrity.
  • (18)F-labeled proteins hold significant promise for clinical diagnostics and therapeutic monitoring.