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SR140333 counteracts NK-1 mediated cell proliferation in human breast cancer cell line T47D
Wei-Qing Huang1, Ji-Gang Wang, Lei Chen
1Department of Pathology, Qingdao Municipal Hospital, 5 Donghai Middle Road, Qingdao, Shandong, 266071, China. chenhua62@163.com.
Background:
It has been demonstrated that certain NK-1 antagonists could reduce proliferation of several cancer cell lines, however, it is unknown whether SR140333 exerts proliferation inhibition in breast cancer cell line.
Methods:
Immunohistochemical staining was carried out to investigate the immunolocation of NK-1 in breast cancer tissues and T47D cell line, thereafter, various concentrations of [Sar9, Met(O2)11]substance P and SR140333 were applied alone or combined. MTT assay was applied to detect cytoactivation and coulter counter was to detect growth curve. The Hoechst33258 staining was performed to detect apoptosis.
Results:
We found that breast cancer and T47D cells bear positive expression of NK-1. SR140333 inhibited cell growth in a dose dependent manner. Furthermore, SR140333 could counteract [Sar9, Met(O2)11]substance P induced proliferation. Hoechst33258 staining revealed the presence of apoptosis after SR140333 treatment.
Conclusions:
Our study demonstrated SR140333 exert proliferation inhibition in breast cancer cell line T47D and indicates NK-1 play a central role in the substance P related cell proliferation in breast cancer.
Insights
SR140333, a neurokinin-1 (NK-1) receptor antagonist, inhibits breast cancer cell growth and induces apoptosis. This NK-1 antagonist demonstrates potential for treating breast cancer by targeting substance P-related proliferation.
Area of Science:
- Oncology
- Cell Biology
- Pharmacology
Background:
- Neurokinin-1 (NK-1) receptor antagonists have shown potential in reducing cancer cell proliferation.
- The specific effect of SR140333 on breast cancer cell lines was previously unknown.
Purpose of the Study:
- To investigate the role of NK-1 in breast cancer.
- To determine if SR140333 inhibits proliferation in breast cancer cell lines.
Main Methods:
- Immunohistochemical staining to detect NK-1 expression in breast cancer tissues and T47D cells.
- MTT assay and Coulter counter to assess cell viability, proliferation, and growth.
- Hoechst33258 staining to evaluate apoptosis induction.
Main Results:
- Breast cancer tissues and T47D cells exhibit positive NK-1 expression.
- SR140333 demonstrated dose-dependent inhibition of cell growth.
- SR140333 counteracted substance P-induced proliferation and induced apoptosis.
Conclusions:
- SR140333 effectively inhibits proliferation in the T47D breast cancer cell line.
- The NK-1 receptor plays a significant role in substance P-mediated breast cancer cell proliferation.
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