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Assessment of oritavancin serum protein binding across species
Francis F Arhin1, Adam Belley, Geoffrey McKay
1The Medicines Company, 7170 Frederick-Banting St., 2nd Floor, St. Laurent, Quebec H4S 2A1, Canada. francis.arhin@themedco.com
Abstract:
Biophysical methods to study the binding of oritavancin, a lipoglycopeptide, to serum protein are confounded by nonspecific drug adsorption to labware surfaces. We assessed oritavancin binding to serum from mouse, rat, dog, and human by a microbiological growth-based method under conditions that allow near-quantitative drug recovery. Protein binding was similar across species, ranging from 81.9% in human serum to 87.1% in dog serum. These estimates support the translation of oritavancin exposure from nonclinical studies to humans.
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