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Related Experiment Video

Updated: Jun 12, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Efficient mRNA detection from human archival paraffin-embedded tissue: an update.

Romana Illig¹, Helga Fritsch, Christoph Schwarzer

¹Department of Anatomy, Division of Clinical and Functional Anatomy, Innsbruck Medical University, 6020 Innsbruck, Austria.

RNA (New York, N.Y.)

|May 26, 2010

Summary

A new in situ hybridization protocol for archival tissue was developed using a defined hybridization buffer. This method ensures reliable results when commercial buffers become unavailable.

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Last Updated: Jun 12, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

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Published on: May 5, 2018

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Published on: August 1, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Histopathology
Molecular Biology
Biotechnology

Background:

Archival tissue samples are crucial for retrospective studies.
In situ hybridization (ISH) is a powerful technique for visualizing nucleic acids in tissues.
The unavailability of a commercial hybridization buffer necessitated the development of an alternative.

Purpose of the Study:

To develop and validate an improved in situ hybridization protocol.
To create a defined hybridization buffer for consistent ISH results.
To provide a reliable method for researchers using archival tissues.

Main Methods:

Optimization of a novel hybridization buffer formulation.
Validation of the protocol using archival tissue samples.
Comparison of results with previous methods.

Main Results:

The developed protocol demonstrated high sensitivity and specificity.
Consistent and reproducible ISH signals were obtained.
The defined buffer effectively replaced the unavailable commercial product.

Conclusions:

The improved ISH protocol with a defined hybridization buffer is effective for archival tissues.
This method offers a robust alternative for ISH applications.
The protocol ensures continued research possibilities with valuable tissue archives.