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Updated: Jun 12, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of
André de Moricz1, Murilo Melo, Ana Maria Castro
1Department of Surgery, Santa Casa School of Medicine, São Paulo, Brazil. amoricz@uol.com.br <amoricz@uol.com.br>
Insights
Helicobacter spp are highly prevalent in chronic cholecystitis. However, the presence of these bacteria, identified by PCR, did not correlate with gallbladder histological findings in this study.
Area of Science:
- Gastroenterology
- Microbiology
- Pathology
Background:
- Chronic cholecystitis is a common condition often associated with gallstones.
- The role of Helicobacter species in gallbladder disease pathogenesis remains under investigation.
- Understanding bacterial involvement can inform diagnostic and therapeutic strategies.
Purpose of the Study:
- To determine the prevalence of Helicobacter species in patients undergoing surgery for chronic cholecystitis.
- To investigate the correlation between Helicobacter species detection and specific histological changes in the gallbladder.
- To compare PCR-based detection with Giemsa staining for identifying Helicobacter in gallbladder tissue.
Main Methods:
- 100 patients with chronic cholecystitis and cholecystolithiasis were analyzed.
- Gallbladder histology was examined for metaplasia, dysplasia, lymphoid follicles, anaplasia, and tumors.
- Helicobacter species DNA was identified using 16S rRNA gene PCR; bacterial presence was also assessed via Giemsa staining.
Main Results:
- Helicobacter species DNA was detected in 61.8% of patients by PCR, while Giemsa staining identified bacteria in 27.9%.
- No significant correlation was found between PCR-positive results for Helicobacter species and histological findings.
- A correlation was observed between Giemsa-positive results and the presence of lymphoid follicles (p=0.025) and metaplasia (p=0.039).
Conclusions:
- A high prevalence of Helicobacter species exists in patients with chronic cholecystitis and cholecystolithiasis.
- The detection of Helicobacter species via PCR in gallbladder bile does not correlate with the studied histological patterns.
- Giemsa staining showed a correlation with specific inflammatory and metaplastic changes, suggesting a potential role in gallbladder pathology.
Purpose:
Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings.
Methods:
100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa(+) by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCRTheta and GiemsaTheta were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05).
Results:
Of 68 patients, 42 (61.8%) were PCR(+) for Helicobacter spp and 19 (27.9%) had Giemsa(+). There was no correlation between the two findings. The PCR(+) for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa(+) (p = 0.025 and p= 0.039).
Conclusion:
There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.
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