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Published on: January 15, 2017
Testicular histology after intestinal pedicle flap (cecum) apposition in rats
Saulo Rodrigues Júnior1, Augusto Diogo Filho, Tânia Machado de Alcântara
1Department of Pediatrics, UFU, Minas Gerais, Brazil. saulorj@prove.ufu.br <saulorj@prove.ufu.br>
Purpose:
Histological study of vascularization between a cecal pedicle flap and the testicle of Wistar rats.
Methods:
Fifty-three rats were studied. G1: submitted to celiotomy (a), mobilization of the right testicle (RT) to the abdomen (b), cecal flap suture to the RT (d) and cavity closure. G1: procedures a, b and d and fixation of RT into abdomen. G3: procedures a, b and d, exposition of RT to air and reposition into scrotum. G4: not operated. Euthanasia and histology was done after 20 days. Histometry and lesions score classification was done. Testicular vascularization was studied with comparison between G1 and G3. A p < .05 was considered significant.
Results:
The G1 RT diameters were not different to G2 RT and all have decreased size in comparison with RT of G3 and G4. The lesions score in the RT was 5.83 in G1 and 3.3 in G2 without statistical difference. The vascularization's average in G1 was 16.9 vessels in 400X field in the RT. In the G3 this average was 0.96 to the RT and 0.92 to left testicles. The weight's average in G1 was similar with G2 but different of G3 and G4.
Conclusion:
A significant increase of vascularization was observed between the intestinal flap and the rat testicle.
