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Updated: Jun 12, 2026
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
Published on: August 25, 2022
Maintenance of antimicrobial susceptibility of Acinetobacter baumannii in modeled microgravity
159th MDW/SGVUL, 59th Clinical Research Division, Lackland AFB, TX 78236-9908, USA. donna.hensley@lackland.af.mil
Objective:
The purpose of this study was to evaluate the effect of a modeled microgravity environment on the antimicrobial resistance of Acinetobacter baumannii.
Design:
Clinical isolates of A. baumannii (n=10) in Mueller Hinton broth were grown in high aspect ratio vessels using a rotary cell culture system oriented to achieve either normal earth gravity (1 x G) or low sheer modeled microgravity (LSMMG) conditions. After culture MICs were determined by Etest. Antimicrobials included in the study were amikacin, ampicillin/sulbactam, azithromycin, ceftazidime, gatifloxacin, gentamicin, imipenem, meropenem, minocycline, ticarcillin/clavulanic acid, tigecycline, and trimethoprim/sulfamethoxazole. Results were compared for MIC value and category interpretation (S, I, R).
Results:
Overall category agreement was 100% (360/360). 100% of MIC values compared were within one twofold dilution of each other.
Conclusion:
Under these test conditions LSMMG had no apparent effect on the resistance pattern of A. baumannii.
