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Updated: Jun 12, 2026
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
[Construction of cDNA library of protozoan ciliates Euplotes octocarinatus]
Baofeng Chai1, Yanqin Hao, Cui Li
1Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China. bfchai@sxu.edu.cn
Unlabelled:
The protein biosynthesis in cells is an open process cooperatively regulated by many protein factors and enzymes, which form a complicated protein network and signal transduction pathway for mRNA metabolism and protein translation.
Objective:
To provide a platform for studying on the function of proteins involving in process of translation termination and the relationship among these proteins in ciliates.
Methods:
We constructed the cDNA library of protozoan ciliates Euplotes octocarinatus strictly following the procedure of BD Matchmaker Library Construction & Screening kit from Clontech.
Results:
We obtained a cDNA library of ciliates Euplotes, the titer of which was about 2.437 x 10(7) cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding RNA helicase.
Conclusion:
This library will provide a convenient platform for identifying the functional genes in ciliates.
