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Updated: Jun 12, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Published on: September 7, 2017
[Mechanism of DNA methylation and demethylation--its role in control of genes expression]
Jolanta Guz1, Marek Foksiński, Ryszard Oliński
1Department of Clinical Biochemistry, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, 24 Karlowicza St., 85-092 Bydgoszcz, Poland. jolaguz@cm.umk.pl
Abstract:
Cytosine methylation is a post-replicative DNA modification associated with transcriptional repression. This process consists in covalent addition of a methyl group to cytosine within the CpG dinucleotide. DNA methylation is catalyzed by DNA methyltransferases which transfer a methyl group from S-adenosyl-L-methionine to cytosine bases in DNA. Methylation patterns are determined by DNA methyltransferases, but also by the process of DNA demethylation. This review describes biochemical aspects of DNA methylation and demethylation and its role in regulation of genes expression, as well as shows cytosine methylation as promising target for anticancer therapy.
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