Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Finding the Right Fit: Caregiver and Clinician Perspectives on Intensive Treatment Schedules for Childhood Apraxia of Speech.

American journal of speech-language pathology·2026
Same author

Correcting curvature in micromirror-based spatial light modulators with a microlens array.

Optics express·2026
Same author

Functional characterization of bat limb regulatory elements.

bioRxiv : the preprint server for biology·2026
Same author

Caregivers' Experiences of Assessment and Treatment for Their Child With Childhood Apraxia of Speech: An International Questionnaire Study.

American journal of speech-language pathology·2026
Same author

Interventions for childhood apraxia of speech.

The Cochrane database of systematic reviews·2026
Same author

Complement-producing adventitial fibroblasts form an IL-33 alarmin hub that maintains ILC2s during airway allergy.

Cell reports·2025

Related Experiment Video

Updated: Jun 12, 2026

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

Seed-based expression systems for plant molecular farming.

Joseph Boothe1, Cory Nykiforuk, Yin Shen

  • 1SemBioSys Genetics Inc, Calgary, Alberta, Canada.

Plant Biotechnology Journal
|May 27, 2010
PubMed
Summary

Seeds offer adaptable and stable platforms for producing recombinant proteins, ideal for biopharmaceutical manufacturing. Their unique properties facilitate cost-effective production and regulatory approval for various therapeutic proteins.

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Area of Science:

  • Plant biotechnology
  • Molecular farming
  • Biopharmaceutical production

Background:

  • Seeds possess inherent properties like dormancy and nutrient storage, offering significant adaptability.
  • These seed characteristics are highly valuable for molecular farming, especially for large-scale recombinant protein production.
  • Transgenic seeds serve as a robust source for extracting and isolating proteins for biopharmaceutical development.

Purpose of the Study:

  • To review factors influencing high-level accumulation of recombinant proteins in seeds.
  • To highlight the critical role of subcellular trafficking and targeting in achieving economically viable protein yields.
  • To discuss the utility of seeds as versatile hosts for diverse recombinant proteins, including complex molecules like antibodies.

Main Methods:

  • Review of genetic elements like promoters and enhancers controlling transcript abundance.
  • Analysis of subcellular targeting mechanisms for polypeptide accumulation.
  • Examination of seed properties (dormancy, low water content) for efficient extraction and cost-effective manufacturing.
  • Case study analysis of specific biopharmaceuticals produced in seeds.

Main Results:

  • Promoters and enhancers are key for transcript abundance, but subcellular targeting is crucial for high-level accumulation.
  • Seeds can host a wide range of recombinant proteins, from simple peptides to complex antibodies.
  • Seed dormancy and low water content simplify long-term storage and cost-effective manufacturing of active pharmaceutical ingredients.
  • Successful preclinical and clinical data exist for seed-produced therapeutics like insulin and human growth hormone.

Conclusions:

  • Seeds are versatile and efficient platforms for producing diverse recombinant proteins for biopharmaceutical applications.
  • Seed properties facilitate stable storage and cost-effective manufacturing, streamlining the path to regulatory approval.
  • Further development of cGMP processes based on seed-derived materials is essential for broader adoption in pharmaceutical production.