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Characterization of cuticular chitin-binding proteins of Leptinotarsa decemlineata (Say) and post-ecdysial transcript
1Potato Research Center, Agriculture and Agri-Food Canada, Fredericton, New Brunswick, Canada.
Abstract:
Seven cuticle chitin-binding proteins (Ld-CP1v1 to 7) were deduced from antenna cDNAs of adult Colorado potato beetles, Leptinotarsa decemlineata (Say), based on their consensus sequences. The mature proteins consisted of 87-188 residues. Ld-CP1v1 formed a distinct orthologous protein cluster (OP1) along with four proteins from other insect species in a neighbor-joining phylogenetic tree. These proteins also contained a proline glutamine-rich (PQ-rich) region and a highly conserved C-terminal motif (Phr). Their consensus region lacked the defined aromatic triad. Ld-CP2 to 6 clustered with those bearing RR-1 consensus and Ld-CP7 with RR-2 consensus. Ld-CP1v1 to 4 were expressed at the post-ecdysial period in all the developmental stages whereas Ld-CP5 to 7 were expressed mainly in adults.
