Related Experiment Video
Updated: Jun 12, 2026
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
NG108-15 hybrid cells take up but do not synthesize serotonin
A S Hilibrand1, U Ingrid Richardson, J K Blusztajn
1Laboratory of Neuroendocrine Regulation, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technoloy, Cambridge, MA 02139, U.S.A.
Abstract:
The mouse neuroblastoma x rat glioma hybrid cell line, NG108-15, does not synthesize serotonin from tryptophan, although the cells take up tryptophan and serotonin in a saturable manner from serum-supplemented incubation medium. Since serum commonly used to supplement the growth medium contains serotonin, it is concluded that appreciable levels of serotonin found in NG108-15 cells are attributable to uptake of serotonin from the serum-supplemented medium.
