An enzymoimmunoassay for rat gamma enolase using two monoclonal antibodies

Monoclonal antibodies specific for rat ? enolase were prepared by fusion between ?? enolase immunized mouse spleen cells and mouse myeloma cells. Isolated hybrid cell lines were screened against both ?? and ?? enolase with an enzymoimmunoassay. Two types of antibodies were identified: one type, corresponding to 21% of the generated clones, recognized both ? and ? subunits, the other type, representing 3% of the clones, was specific for ? enolase. The specificity of antibodies was ascertained by immunofixation of ?? enolase activity. Two monoclonal antibodies (2H12 and 8A9) were used for quantitative determination of rat ? enolase in a "sandwich type" enzymoimmunoassay. The ? enolase has been shown to be a specific neuronal and neuroendocrine product. The development of a ? enolase assay will facilitate the study of the regulation of ? enolase expression.