Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The solution structure of human transforming growth factor alpha.

T S Harvey1, A J Wilkinson, M J Tappin

  • 1Department of Biochemistry, University of Oxford, England.

European Journal of Biochemistry
|June 15, 1991
PubMed
Summary

This study determined the solution structure of transforming growth factor alpha using NMR and computational methods. The findings offer insights into its conformational changes and pH-dependent behavior.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lessons from COVID-19 for behavioural and communication interventions to enhance vaccine uptake.

Communications psychology·2024
Same author

Fact Check: Assessing the Response of ChatGPT to Alzheimer's Disease Myths.

Journal of the American Medical Directors Association·2024
Same author

Fact Check: Assessing the Response of ChatGPT to Alzheimer's Disease Statements with Varying Degrees of Misinformation.

medRxiv : the preprint server for health sciences·2023
Same author

COVID-19, Framing and Naming a Pandemic: How What Is Not in a Disease Name May Be More Important than What Is.

Pathogens (Basel, Switzerland)·2023
Same author

Pupils returning to primary schools in England during 2020: rapid estimations of punctual COVID-19 infection rates.

Royal Society open science·2021
Same author

A novel approach for evaluating contact patterns and risk mitigation strategies for COVID-19 in English primary schools with application of structured expert judgement.

Royal Society open science·2021

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Dynamics

Background:

  • Transforming growth factor alpha (TGF-α) is a key signaling protein.
  • Understanding its solution structure is crucial for elucidating its biological functions.

Purpose of the Study:

  • To determine the high-resolution solution structure of transforming growth factor alpha.
  • To investigate the pH-dependent conformational interconversion of TGF-α.

Main Methods:

  • Utilized high-resolution 1H-nuclear magnetic resonance (NMR) spectroscopy.
  • Employed distance geometry and restrained molecular dynamics for structure calculation.
  • Refined structures using restrained molecular mechanics and various other methods.

Main Results:

Related Experiment Videos

  • Generated a set of 382 restraints from NMR experiments.
  • Calculated and refined multiple TGF-α structures.
  • Evaluated structure accuracy using hydrogen bonding patterns and amide exchange rates.
  • Proposed mechanisms for observed pH-dependent conformational changes.

Conclusions:

  • The study successfully elucidated the solution structure of TGF-α.
  • Insights into TGF-α's structural dynamics and pH sensitivity were gained.
  • This work provides a foundation for further research on TGF-α interactions and functions.