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Published on: May 10, 2017
[Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells]
Dan Zhang1, Shao-xi Cai, Hai-jin Zhao
1Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. winterpower@163.com
Objective:
To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE).
Methods:
16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively.
Results:
Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation.
Conclusion:
Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.
