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Updated: Jun 12, 2026
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
[Construction of human full-length renal cell carcinoma patient-specific antibody library by mammalian cell surface
Zhen-rui Chen1, Chang-zheng Li, Wei He
1Department of Urology, Nanfang Hospital, School of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China. chenzhenrui.surgeon@gmail.com
Objective:
To construct human renal cell carcinoma patient-specific full-length antibody library using mammalian cell surface display technique.
Methods:
Peripheral blood mononuclear cells (PBMC) were isolated from patients with renal cell carcinoma. The repertoires of kappa light chain (LCkappa) and heavy chain variable region (VH) of antibody were amplified by RT-PCR. The LCkappa and VH libraries were inserted into the vector pDGB-HC-TM separately, and the ligated libraries were transformed into competent E.coli TOPO10 to construct the renal cell carcinoma patient-specific antibody heavy and light chain libraries. 293T cells were co-transfected with the libraries and the full-length human antibodies expressed on the surface of 293T cells were analyzed by flow cytometry.
Results:
The libraries of renal cell carcinoma-specific antibody kappa light chain (LCkappa) and heavy chain (IgG1) were constructed. The expression of the full-length human antibodies on the surface of 293T cell was confirmed by flow cytometry. The libraries showed an expressible combinatory diversity of 7.5x10(10).
Conclusion:
The expressible antibody library provides a useful platform for screening of renal cell carcinoma-specific antibodies.
