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Updated: Jun 12, 2026

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
Published on: January 22, 2020
Levels of semenogelin in human spermatozoa decrease during capacitation: involvement of reactive oxygen species and
1Urology Research Laboratory, H6.47, McGill University Health Centre at Royal Victoria Hospital, 687 Ave des Pins ouest, Montréal, QC, Canada H3A 1A1. edelamirande@yahoo.com
Semenogelin (Sg) prevents sperm capacitation by inhibiting reactive oxygen species (ROS) generation. Spermatozoa internalize and degrade Sg in a zinc-dependent manner, which may allow capacitation to begin.
Area of Science:
- Reproductive Biology
- Sperm Physiology
- Protein Biochemistry
Background:
- Semenogelin (Sg) is the primary protein in human semen coagulum.
- Sg plays a crucial role in inhibiting sperm capacitation.
Purpose of the Study:
- To investigate the role of Semenogelin (Sg) in sperm function.
- To elucidate the mechanism by which Sg affects sperm capacitation.
Main Methods:
- Sperm capacitation was induced by various stimuli.
- Inhibition of superoxide anion and nitric oxide generation was measured.
- Sg and its peptides were detected using immunoblotting.
- Immunocytochemistry was used to assess Sg localization on spermatozoa.
- Spermatozoa internalization and degradation of Sg were analyzed.
Main Results:
- Sg inhibited sperm capacitation by blocking superoxide anion and nitric oxide generation.
- Sg levels decreased during capacitation, a process influenced by reactive oxygen species (ROS) and zinc.
- Spermatozoa internalized and degraded Sg, with degradation being inhibited by zinc.
Conclusions:
- Semenogelin (Sg) primarily blocks sperm capacitation through the inhibition of ROS generation.
- Spermatozoa can internalize and degrade Sg, a process that is modulated by zinc.
- ROS-induced degradation of Sg may be a key mechanism initiating sperm capacitation.
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