Related Experiment Video

Updated: Jun 12, 2026

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

The deubiquitinating enzyme USP26 is a regulator of androgen receptor signaling

Annette M G Dirac1, René Bernards

  • 1The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, 1066 CX Netherlands. a.dirac@nki.nl

Molecular Cancer Research : MCR
|May 27, 2010
PubMed

Insights

The deubiquitination enzyme USP26 regulates androgen receptor (AR) signaling by modulating AR ubiquitination, influencing its activity and stability. This finding offers new insights into AR regulation, crucial for male development and prostate cancer.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Oncology

Background:

  • The androgen receptor (AR) is a nuclear receptor vital for male sexual development and prostate cancer.
  • AR signaling is regulated by posttranslational modifications, including ubiquitination.

Purpose of the Study:

  • To identify novel regulators of androgen receptor (AR) signaling.
  • To investigate the role of deubiquitination enzymes in AR regulation.

Main Methods:

  • Screened a short hairpin library targeting deubiquitination enzymes.
  • Investigated the interaction between USP26 and AR.
  • Assessed the impact of USP26 on AR ubiquitination, activity, and stability.

Main Results:

  • Identified USP26 as a novel regulator of AR signaling.
  • USP26 binds to AR and modulates its ubiquitination.
  • USP26 counteracts hormone-induced AR ubiquitination, affecting AR transcriptional activity and stability.

Conclusions:

  • USP26 is a nuclear protein that regulates AR signaling through ubiquitination.
  • USP26 plays a role in AR stability and transcriptional activity.
  • USP26 represents a potential therapeutic target for AR-dependent diseases like prostate cancer.

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