Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spinally mediated opioid antidiarrheal effects.

P K Lemcke1, J E Shook, T F Burks

  • 1Department of Pharmacology, College of Medicine, University of Arizona Health Sciences Center, Tucson 85724.

European Journal of Pharmacology
|January 25, 1991
PubMed
Summary

Spinal cord opioid receptors play a role in regulating intestinal mucosa function. Selective mu (PL017) and delta (DPDPE) opioid agonists administered intrathecally (i.t.) effectively inhibited prostaglandin E2 (PGE2)-induced diarrhea in mice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shock and vibration data acquisition system for off-road vehicle operator health and safety assessment.

Journal of agricultural safety and health·2006
Same author

Infectious diseases in the emergency setting Part 1. Introduction.

Seminars in pediatric infectious diseases·2006
Same author

Infection control in the emergency department.

Seminars in pediatric infectious diseases·2006
Same author

Distribution fitting and parameterization of individual operator work routine times for small dairy parlors.

Journal of dairy science·2006
Same author

New agents for the treatment of cancer-related fatigue.

Cancer·2001
Same author

Comparison of intravenous midazolam with pentobarbital for sedation for head computed tomography imaging.

Academic emergency medicine : official journal of the Society for Academic Emergency Medicine·2000

Area of Science:

  • Neuroscience
  • Gastroenterology
  • Pharmacology

Background:

  • Opioid receptors are known to modulate gastrointestinal functions.
  • The role of spinal opioid receptors in regulating intestinal mucosal secretion, specifically in diarrhea, requires further investigation.

Purpose of the Study:

  • To investigate the role of spinal opioid receptors in the regulation of intestinal mucosal function.
  • To assess the antidiarrheal effects of selective mu, delta, and kappa opioid agonists administered intrathecally (i.t.) in a mouse model of prostaglandin E2 (PGE2)-induced diarrhea.

Main Methods:

  • Selective mu (PL017), delta (DPDPE), and kappa (U50,488H) opioid agonists were administered intrathecally (i.t.) in mice.
  • Diarrhea was induced by intraperitoneal (i.p.) injection of prostaglandin E2 (PGE2).

Related Experiment Videos

  • Antidiarrheal effects were assessed in a dose-related manner, and compared to antinociception and gastrointestinal transit inhibition.

    • Main Results:

    • Intrathecal administration of mu (PL017) and delta (DPDPE) opioid agonists dose-dependently inhibited PGE2-induced diarrhea.
    • Kappa opioid agonist (U50,488H) showed minimal antidiarrheal effects.
    • Spinal opioid administration was less potent than intracerebroventricular (i.c.v.) but more effective than peripheral (s.c.) administration.
    • Antidiarrheal effects were antagonized by naloxone, confirming opioid receptor involvement.

    Conclusions:

    • Opioid chemosensitive sites within the spinal cord can modulate diarrhea induced by PGE2.
    • Selective mu and delta opioid agonists possess antidiarrheal activity when administered intrathecally.
    • The spinal cord represents a potential target for antidiarrheal therapies involving opioid receptor modulation.