Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypochlorite Accident in a Pediatric Patient.

Kathmandu University medical journal (KUMJ)·2024
Same author

A systematic review and meta-analysis of spectral CT to differentiate focal liver lesions.

Clinical radiology·2023
Same author

Reactions in leprosy patients triggered by COVID-19 vaccination - a cross-sectional study from a tertiary care centre in India.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Role of Magnetic Resonance Cholangiopancreatography in the Evaluation of Obstructive Jaundice.

Kathmandu University medical journal (KUMJ)·2021
Same author

Central vein sign for multiple sclerosis: A systematic review and meta-analysis.

Clinical radiology·2020
Same author

Center of pressure metrics derived from spatially registered typically developing data.

Gait & posture·2019

Related Experiment Video

Updated: Jun 12, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

DNA as therapeutics; an update.

P Saraswat1, R R Soni, A Bhandari

  • 1Mahatma Gandhi Medical College and Hospital, RIICO Institutional Area, Sitapura, Jaipur-302 022, India.

Indian Journal of Pharmaceutical Sciences
|May 27, 2010
PubMed
Summary
This summary is machine-generated.

Human gene therapy introduces genetic material to treat diseases like cancer and genetic disorders. This review covers DNA-based therapeutics and gene transfer technologies, exploring current advancements and future potential.

Keywords:
DNA delivery systemsGene therapyantisensegene therapy trialsgene transfer technologyliposomesnonviral vectorsnucleic acid therapeuticsviral vectors

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Related Experiment Videos

Last Updated: Jun 12, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Human gene therapy involves introducing genetic material (DNA and RNA) into cells for therapeutic purposes.
  • It holds potential for treating numerous diseases, including cancers, acquired immunodeficiency syndrome, and various genetic disorders.
  • Gene therapy research is advancing, with ongoing trials and developing technologies.

Purpose of the Study:

  • To review deoxyribonucleic acid (DNA)-based therapeutics and gene transfer technologies.
  • To summarize the current status and recent developments in human gene therapy research.
  • To discuss the potential of gene therapy in combating genetic and other diseases.

Main Methods:

  • Review of existing literature on DNA-based therapeutics and gene transfer.
  • Analysis of current gene therapy research and clinical trial data.
  • Summarization of therapeutic approaches including plasmids, oligonucleotides, aptamers, and DNAzymes.

Main Results:

  • DNA-based therapeutics encompass plasmids, oligonucleotides, aptamers, and DNAzymes.
  • Gene therapy utilizes both somatic and germ line approaches.
  • Significant progress has been made in gene transfer technologies and understanding genetic diseases.

Conclusions:

  • Human gene therapy, utilizing DNA and RNA, shows promise as a future medical treatment.
  • Continued research and ethical considerations are crucial for the advancement of gene therapy.
  • Gene therapy offers a potential new avenue for treating a wide range of debilitating conditions.