Simultaneous RP-HPLC Estimation of Trifluoperazine Hydrochloride and Chlordiazepoxide in Tablet Dosage Forms

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Accurate quantification of drug mixtures is essential for pharmaceutical quality control.
• Trifluoperazine hydrochloride and chlordiazepoxide are commonly used therapeutic agents whose combined analysis requires robust methods.

Purpose of the Study:

• To develop and validate a reversed-phase liquid chromatography (RPLC) method for the simultaneous determination of trifluoperazine HCl and chlordiazepoxide.
• To assess the applicability of the developed RPLC method for analyzing these drugs in tablet dosage forms.

Main Methods:

• Utilized reversed-phase liquid chromatography with a methanol:water (97:03, v/v) mobile phase.
• Employed ultraviolet detection at 262 nm for quantification.
• Validated the method over concentration ranges of 0.1-1 µg/ml and 0.5-5 µg/ml.

Main Results:

• Achieved high accuracy, with mean values of 101.05±0.47% and 98.97±0.33% for the respective concentration ranges.
• Demonstrated successful application to tablet formulations without interference from excipients.
• Confirmed method reliability through the standard addition technique, showing consistent accuracy and precision.

Conclusions:

• The developed RPLC method is accurate, precise, and suitable for the routine quality control of trifluoperazine HCl and chlordiazepoxide in pharmaceutical preparations.
• The method's robustness and lack of interference from common tablet excipients make it a valuable analytical tool.