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Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora.

Li-Chuan Chuang¹, Chiung-Shing Huang, Li-Wei Ou-Yang

¹Department of Pediatric Dentistry, Chang Gung Memorial Hospital (Linkou), No. 5, Fusing St., Gueishan Township, Taoyuan County 333, Taiwan, Republic of China.

Clinical Oral Investigations

|May 27, 2010

Summary

A novel probiotic, Lactobacillus paracasei GMNL-33, showed a delayed effect in reducing Streptococcus mutans counts in saliva. Significant reductions were observed two weeks after a two-week oral tablet treatment in healthy adults.

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Area of Science:

Microbiology
Oral Health
Probiotics

Background:

Lactobacillus paracasei demonstrates in vitro inhibition of pathogenic microbes like Streptococcus mutans.
Clinical efficacy of L. paracasei for oral health applications requires further investigation.

Purpose of the Study:

To evaluate the effect of a novel probiotic, L. paracasei GMNL-33, on salivary microbial counts associated with dental caries in healthy adults.

Main Methods:

A double-blinded, randomized, placebo-controlled study involving 78 healthy adults (aged 20-26).
Participants received either L. paracasei GMNL-33 or a placebo oral tablet three times daily for two weeks.
Salivary counts of S. mutans and lactobacilli, along with buffer capacity, were measured at baseline, post-treatment, and two weeks after treatment.

Main Results:

No significant differences in S. mutans or lactobacilli counts were observed between the probiotic and placebo groups at any measurement point.
Within the probiotic group, a significant reduction in salivary S. mutans counts was noted between the end of treatment and the two-week follow-up (p=0.016).
No immediate inhibitory effect on S. mutans was detected during the two-week treatment period.

Conclusions:

A two-week oral administration of L. paracasei GMNL-33 may require an additional two weeks post-treatment to demonstrate a significant reduction in salivary Streptococcus mutans.
The findings suggest a delayed probiotic action of L. paracasei GMNL-33 on caries-associated bacteria.