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A simplified method for screening the apolipoprotein E polymorphism
J E Eichner1, L H Kuller, R E Ferrell
1Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pa.
Human Heredity
|January 1, 1991
Abstract:
A simple method for screening populations for the apolipoprotein E polymorphism which involves isoelectric focusing of delipidated samples on polyacrylamide gels of pH 4.5-5.8 followed by immunoblotting using a double-antibody technique is presented. The method is a synthesis of two previously published procedures and works well on samples that have been stored for as long as 15 years.