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Updated: Jun 12, 2026

Intracranial Pharmacotherapy and Pain Assays in Rodents
Published on: April 9, 2019
Na(+), K(+) ATPase activity in membrane fractions of rat central and peripheral nervous tissues modified by opioids
1Institute of Physiological Regulations, Czechoslovak Academy of Sciences, Bulovka, Pavilion 11, 180 85 Prague 8, Czechoslovakia.
Abstract:
The inhibition by ouabain of Na(+), K(+) ATPase activity in lumbar spinal cord membranes followed a sigmoid curve and in sciatic nerve membranes an exponential curve. The degree of inhibition in spinal cord depended on the presence of opioid ligands; fractions became more sensitive to ouabain after removal of bound opioids. The saturation of receptors by specific ligands reversed the effect of preincubation. Opioids had no effect on Na(+), K(+) ATPase in sciatic nerve. Membranes isolated from spinal cord were highly sensitive to oubain inhibition and their Na(+), K(+) ATPase resembled that containing the ? (+) subunit; membranes from peripheral nerve were less sensitive to ouabain and their enzyme reacted as that containing the ? subunit.
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