Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 12, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

[(3)H]Minaprine binding to membrane preparation from rat brain.

M Muramatsu1, C Usuki-Ito, H Aihara

  • 1Department of Pharmacology, Research Center, Taisho Pharmaceutical Co. Ltd, 403 Yoshino-cho 1-chome, Ohmiya, Saitama 330, Japan.

Neurochemistry International
|May 28, 2010
PubMed
Summary

Minaprine binds to a specific site in the rat brain, primarily on synaptic membranes. This binding, particularly in the hippocampus, suggests a link to voltage-dependent potassium channels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of the Radiative Decay D_{s0}^{*}(2317)^{+}→D_{s}^{*+}γ.

Physical review letters·2026
Same author

First Search for B→X_{s}νν[over ¯] Decays.

Physical review letters·2026
Same author

Search for Feebly Interacting Particles in B Decays with Missing Energy at Belle.

Physical review letters·2026
Same author

Search for B^{0}→K^{*0}τ^{+}τ^{-} Decays at the Belle II Experiment.

Physical review letters·2025
Same author

Search for a Dark Higgs Boson Produced in Association with Inelastic Dark Matter at the Belle II Experiment.

Physical review letters·2025
Same author

Study of χ_{bJ}(2P)→ωϒ(1S) at Belle.

Physical review letters·2025

Area of Science:

  • Neuropharmacology
  • Molecular Neuroscience
  • Drug Discovery

Background:

  • Minaprine is a drug with potential neurological applications.
  • Understanding its specific binding sites is crucial for elucidating its mechanism of action.
  • Previous studies have not fully characterized minaprine's central nervous system targets.

Purpose of the Study:

  • To identify and characterize the specific binding site of minaprine in the rat brain.
  • To determine the affinity and distribution of minaprine binding.
  • To investigate the molecular nature of the minaprine binding site.

Main Methods:

  • Radioligand binding assays using [3H]minaprine in various rat brain fractions (synaptosomal, mitochondrial, microsomal).
  • Determination of dissociation constants (Kd) and distribution studies across brain regions (hippocampus, cerebral cortex, striatum).

More Related Videos

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
09:49

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Published on: May 12, 2017

Related Experiment Videos

Last Updated: Jun 12, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
09:49

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Published on: May 12, 2017

  • Investigation of binding thermodynamics and inhibition by related compounds and neurotransmitters.

    • Main Results:

    • [3H]minaprine binding was highest in the hippocampus (Kd = 56.3 nM) and predominantly located in the synaptosomal fraction.
    • Binding affinity was influenced by temperature, with thermodynamic analysis indicating favorable entropy changes.
    • Minaprine and its metabolite M-3 inhibited binding, while phencyclidine, tetraethylammonium (TEA), and 4-aminopyridine showed some inhibitory effects, unlike other neurotransmitters.

    Conclusions:

    • Minaprine exhibits high-affinity binding to a specific site on rat brain synaptic membranes.
    • The binding site appears to be associated with voltage-dependent potassium (K+) channels.
    • These findings provide insights into minaprine's neuropharmacological profile and potential therapeutic targets.