Related Experiment Video
Updated: Jun 12, 2026

Using Live-Cell Imaging to Measure the Effects of Pathological Proteins on Axonal Transport in Primary Hippocampal Neurons
Published on: December 22, 2023
Slow anterograde axonal transport of calpain I and II
1Institute of Neurobiology, University of Göteborg, P.O. Box 33 031, S-400 33 Göteborg, Sweden.
Abstract:
Following intravitreal injections of (35)S-amino acids to label axonally transported proteins, calpain I and II from the superior colliculi and the lateral geniculate bodies were isolated via hydrophobic interaction chromatography, anion exchange HPLC and sodium-dodecyl sulphate electrophoresis. Among the slowly transported proteins radioactive label was associated with molecular weight components corresponding to both the light (approx. 30 kDa) and the heavy (approx. 80 kDa) subunits of calpain I as well as with the corresponding subunits of calpain II. Among the fast transported material no radioactive protein bands were detected in these fractions.
Related Concept Videos
Neurons: The Axon
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment.
The Movement of Organelles and Vesicles
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Relaxation of Skeletal Muscles
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
