Related Experiment Video
Updated: Jun 12, 2026

Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking
Published on: March 28, 2014
Pharmacologically separable [(3)H]glutamate binding sites on insect muscle membranes
1AFRC Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing St, Cambridge CB2 3EJ, U.K.
Abstract:
A high affinity, saturable, [(3)H]l-glutamate binding site has been described in a cockroach (Periplaneta americana) coxal muscle membrane preparation. This site exhibits a K(D) of 1.1 ?M and a B(max) of 15.5 pmol mg(?1) protein. The pharmacological profile is compatible with that expected of a functional l-glutamate receptor and there is good agreement with physiological data obtained for the insect neuromuscular junction in other laboratories. There appear to be at least two subpopulations of l-glutamate binding sites in cockroach muscle membranes which can be distinguished biochemically with the agonists ibotenate and quisqualate.
More Related Videos
09:49Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
Published on: April 15, 2016
04:48A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate
Published on: July 10, 2018
Related Concept Videos
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Indirect-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...
Neuromuscular Junction And Blockade
Nondepolarizing (Competitive) Neuromuscular Blockers: Mechanism of Action
Competitive antagonists prevent acetylcholine from binding to its receptor, inhibiting membrane depolarization. Without conformational changes or intrinsic...
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...
Ligand-gated Ion Channels
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...