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Related Experiment Video

Updated: Jun 12, 2026

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
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Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Published on: August 25, 2022

Simulated microgravity-induced epigenetic changes in human lymphocytes.

Kamaleshwar P Singh1, Ragini Kumari, James W Dumond

  • 1Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, Texas 79409, USA. kamaleshwar.singh@tiehh.ttu.edu

Journal of Cellular Biochemistry
|May 28, 2010
PubMed
Summary

Simulated microgravity alters DNA methylation and gene expression in human T-lymphocytes. These epigenetic changes, including DNA hypomethylation, may explain adverse health effects during space flight.

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Area of Science:

  • Space Biology
  • Epigenetics
  • Cellular Biology

Background:

  • Space flight and simulated microgravity alter cellular functions through unknown mechanisms.
  • Epigenetic modifications like DNA methylation and histone acetylation regulate gene expression.

Purpose of the Study:

  • Investigate microgravity's effects on DNA methylation and histone acetylation.
  • Examine the expression of key epigenetic regulator genes (DNMT1, DNMT3a, DNMT3b, HDAC1).

Main Methods:

  • Human T-lymphocytes cultured in a rotary cell culture system (RCCS) simulating microgravity.
  • DNA methylation assessed using methylation sensitive-random amplified polymorphic DNA (MS-RAPD).
  • Gene expression quantified via Quantitative Real-time PCR.

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Last Updated: Jun 12, 2026

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
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In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
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Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
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Published on: January 13, 2019

Main Results:

  • Microgravity increased DNMT1, DNMT3a, and DNMT3b expression at 72h, decreasing by 7 days.
  • Simulated microgravity induced DNA hypomethylation and mutations.
  • HDAC1 expression decreased over time; acetylated histone H3 levels also decreased, suggesting involvement of other HDACs.

Conclusions:

  • Epigenetic alterations, including DNA hypomethylation and changes in DNMT and HDAC expression, are implicated in microgravity-induced gene expression changes.
  • These findings suggest epigenetic mechanisms contribute to adverse health effects in microgravity environments.