Related Experiment Video
Updated: Jun 12, 2026
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Published on: April 1, 2014
[Sequential preparation of microvlllous and basal membranes from human placenta]
Ning Long1, Ai-yun Xing, Xiao-hua Yang
1Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To improve the technology of isolating paired fractions of the maternal-facing membranes (MVM) and fetal-facing plasma membranes (BM) from a term placenta.
Methods:
The component of buffer was improved based on Illsley method. The time of Mg2+ -aggregated basal membranes was extended. MVM were obtained from the supernatant of low speed centrifugation while BM were further purified on a sucrose step gradient.
Results:
Yield for MVM and BM prepared by the method were (0.55 +/- 10.10) mg/g and (0.54 +/- 0.02) mg/g wet weight of placenta. They were enriched 16.87-fold and 11.19-fold as determined by the membrane marker enzymes, alkaline phosphatase (MVM) and adenylate cyclase (BM).
Conclusion:
The modified Illsley method can easily produce both MVM and BM of satisfied quantity from human placenta. It could be applied as a cell molecular model of maternal-fetal exchange interface.
