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[rDNA ITS sequence analysis of wild Gentiana from Gansu province]
Lisha Liu1, Xiangmei Wang, Xin Wang
1Gansu College of Traditional Chinese Medicine, Department of Basic Courses, Lanzhou 730000, China. liulisha@gszy.edu.cn
Objective:
To find the patterns of the rDNA ITS sequence variation in Gentiana, and establish the molecular biological method for the identification of the four kinds wild Gentiana from different regions in Gansu.
Method:
The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of Clustal X, MEGA3.
Result:
The Complete ITS sequence of G. macrophylla, G. straminea, G. dahurica and G. officinale was 800 bp. The sequences of ITS1, ITS2 and 5.8S were 290, 340, 170 bp, respectively. Phylogenetic tree based on ITS1 and ITS2 sequences data was constrcuted by Neighbor-joining method.
Conclusion:
ITS sequence could be as the evidence for the molecular authentication of Gentiana.
