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Updated: Jun 12, 2026
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
[In vitro activity of baicalin against non-albicans Candida biofilms]
Changzhong Wang1, Huijuan Cheng, Xiongfei Zhang
1School of Integrated Traditional and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China. ahwcz63@sina.com
Objective:
To investigate the effects of baicalin against Candida glabrata, C. parapsilosis, C. krusei and C. guilliermondii biofilms.
Method:
96-well microtitre plates were used to set up the biofilms; microdilution method was applied to detect minimal inhibitory concentration (MIC) of baicalin for the four non-albians Candida, and XTT reduction assay was adopted to determine sessile minimal inhibitory concentration (SMIC) of baicalin against the four isolates and to detect the effects on adhesion of the fungal cells.
Result:
MICs of baicalin for the four non-albians Candida cells were 125, 250, 125, 62.5 mg L(-1), respectively. The four non-albians Candida could form mature biofilms on 96-well microtitre plates. SMIC50 of baicalin for the four isolates were > 1000, 500, 125, 250 mg x L(-1), respectively. SMIC80 for the four isolates were greater than or equal to 1000 mg x L(-1). Baicalin showed potent inhibitory effects on adhesion of the four non-albians Candida cells.
Conclusion:
Baicalin displays substantial inhibitory effects on C. parapsilosis, C. krusei and C. guilliermondii biofilm.
