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Updated: Jun 12, 2026

Method of Studying Palatal Fusion using Static Organ Culture
04:58

Method of Studying Palatal Fusion using Static Organ Culture

Published on: September 19, 2015

The ideal timing for experimental cleft lip creation.

Jessico M Leitão, Luis A V Pereira, Frances L L Gonçalves

    The Cleft Palate-Craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association
    |May 29, 2010
    PubMed
    Summary

    Researchers identified the optimal gestational age for creating lip defects in rat models to study cleft lip and palate (CLP). Surgical creation at 19.5 days of gestation yielded the most accurate lip defect model for CLP research.

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    Area of Science:

    • Developmental Biology
    • Craniofacial Development
    • Animal Models in Research

    Background:

    • Cleft lip and palate (CLP) represents the most frequent congenital facial defect.
    • Animal models are crucial for understanding CLP embryogenesis and pathogenesis.
    • Optimal timing for surgical induction of lip defects in rat models remains undefined.

    Purpose of the Study:

    • To refine experimental models for CLP research using rat fetuses.
    • To determine the most suitable gestational age for surgically creating lip defects in rats.

    Main Methods:

    • Surgical creation of a left-sided superior lip defect in rat fetuses.
    • Three experimental groups were established, with procedures performed at 17.5, 18.5, and 19.5 days of gestation.
    • Fetuses were harvested at 21.5 days of gestation for macroscopic and microscopic evaluation.

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    Last Updated: Jun 12, 2026

    Method of Studying Palatal Fusion using Static Organ Culture
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    Method of Studying Palatal Fusion using Static Organ Culture

    Published on: September 19, 2015

    Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
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    Main Results:

    • Lip defect creation at 19.5 days of gestation resulted in significant lip depression.
    • This timing led to observable asymmetry and retraction at the defect site.
    • Complete reepithelialization of the defect borders was noted at 19.5 days gestation.

    Conclusions:

    • The gestational age of 19.5 days is identified as the most appropriate for surgical induction of lip defects in rat models.
    • This timing facilitates the creation of a reliable model for studying cleft lip and palate.
    • The observed lip depression, asymmetry, and reepithelialization support the utility of this model.