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Related Experiment Video

Updated: Jun 12, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

Potato virus X: structure, disassembly and reconstitution.

Joseph Atabekov¹, Eugen Dobrov, Olga Karpova

¹Department of Virology and A.N. Belozersky Institute of Physico-Chemical Biology of Moscow State University, Moscow 119992, Russia.

Molecular Plant Pathology

|May 29, 2010

Summary

This study reveals the structural details of Potato virus X (PVX) coat protein and its disassembly mechanisms. Findings suggest a novel

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In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

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Published on: March 27, 2016

Area of Science:

Plant virology
Molecular biology
Structural biology

Background:

Potato virus X (PVX) is a flexuous filamentous plant potexvirus.
Understanding PVX structure is crucial for controlling its spread.

Purpose of the Study:

To elucidate the tertiary structure of PVX coat protein (CP).
To investigate PVX virion disassembly mechanisms.
To explore the role of post-translational modifications in PVX.

Main Methods:

Tritium planigraphy and protein tertiary structure prediction for CP modeling.
Analysis of PVX virion disassembly triggered by factors including the movement protein (TGBp1).
Experiments on PVX CP repolymerization and reconstitution.

Main Results:

A model for PVX CP tertiary structure in the virion was proposed.
Two PVX disassembly pathways were identified, one involving TGBp1.
Translatable 'RNA-CP-TGBp1' complexes were observed, potentially representing the viral transport form.

Conclusions:

Glycosylation and phosphorylation may influence PVX CP structure and function.
TGBp1 binding destabilizes the virion, facilitating RNA release and translation.
The identified complexes offer insights into PVX infection and transport.