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Determination of 3-methylhistidine in hydrolysed proteins by fluorescamine derivatization and high-performance liquid
1CNR Unit Institute of General Pathology, University of Padova, Italy.
Journal of Chromatography
|January 4, 1991
Abstract:
A method is described for the determination of the 3-methylhistidine content in myofibrilar proteins (myosin and actin) using reversed-phase high-performance liquid chromatography with ultraviolet detection. Proteins were hydrolysed and free amino acids were derivatized with fluorescamine. Elution was performed isocratically with acetonitrile-water (24:76). This method allows the detection of picomole amounts of 3-methylhistidine in myosin and actin.