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Related Concept Videos

Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:13

Protein Organization

Overview
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jun 12, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Integrative structure modeling of macromolecular assemblies from proteomics data.

Keren Lasker1, Jeremy L Phillips, Daniel Russel

  • 1Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California 94158, USA. kerenl@salilab.org

Molecular & Cellular Proteomics : MCP
|May 29, 2010
PubMed
Summary

Understanding protein interactions is key to discovering functional protein complexes. Integrative computational methods combine proteomics and structural data to model these complexes, from composition to atomic detail.

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Last Updated: Jun 12, 2026

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • Proteomics techniques generate extensive protein interaction data.
  • Knowledge gaps exist regarding the formation of functional multiprotein complexes from these interactions.

Purpose of the Study:

  • To bridge the gap between protein interaction data and functional complex structures.
  • To develop integrative computational methods for describing protein complexes.

Main Methods:

  • Combining data from proteomics experiments.
  • Integrating data from structure determination techniques.
  • Developing integrative computational methods.

Main Results:

  • Methods provide descriptions of protein complexes.
  • Varying levels of accuracy and resolution are achieved.
  • Descriptions range from complex compositions to detailed atomic structures.

Conclusions:

  • Integrative approaches are crucial for understanding protein complex formation.
  • Computational modeling enhances the interpretation of proteomics data.
  • This research advances the structural and functional characterization of protein complexes.