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Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...

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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
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MHC I stabilizing potential of computer-designed octapeptides.

Joanna M Wisniewska1, Natalie Jäger, Anja Freier

  • 1Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-Universität, Siesmayerstrasse. 70, 60323 Frankfurt am Main, Germany.

Journal of Biomedicine & Biotechnology
|May 29, 2010
PubMed
Summary

Computational design identified nine stabilizing octapeptides for the H-2K(b) molecule. Results show canonical sequence motifs alone are insufficient for predicting major histocompatibility complex I protein stabilization.

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Area of Science:

  • Immunology and Computational Biology

Background:

  • Major histocompatibility complex (MHC) class I molecules present peptides to T cells, crucial for immune response.
  • Designing peptides that stabilize MHC class I molecules is important for immunotherapy and vaccine development.

Purpose of the Study:

  • To computationally design and experimentally validate octapeptide sequences for stabilizing the H-2K(b) MHC class I molecule.
  • To investigate the predictive power of canonical sequence motifs for MHC class I stabilization.

Main Methods:

  • In silico design of 180 octapeptide sequences using ant colony optimization and artificial neural networks.
  • Experimental validation of designed peptides for their H-2K(b) stabilizing effects.
  • Sequence space visualization using self-organizing maps for 100,603 octapeptides.

Main Results:

  • Nine out of 180 designed octapeptides demonstrated stabilizing effects on H-2K(b).
  • 171 designed peptides did not stabilize H-2K(b), including 28 with known favorable motif residues.
  • Analysis revealed that canonical sequence motifs alone are insufficient predictors of MHC class I stabilization.

Conclusions:

  • The combination of computational design algorithms and experimental validation is effective for identifying MHC class I stabilizing peptides.
  • Relying solely on established canonical motifs from databases like SYFPEITHI is inadequate for predicting MHC class I stabilization.
  • Further exploration of sequence space beyond traditional motifs is necessary for optimizing peptide design for MHC class I molecules.