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Updated: Jun 12, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens.

Hiroya Yamada¹, Rie Maruo, Mikio Watanabe

¹Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Biochemical and Biophysical Research Communications

|June 1, 2010

Summary

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This study introduces FACS-mQ, a new method for sorting and analyzing cells based on intracellular markers, enabling RNA analysis without cell death. This technique is crucial for detecting rare stem cells and cancer stem cells.

Area of Science:

Biotechnology
Cell Biology
Molecular Biology

Background:

Detecting rare cell populations like stem cells and cancer stem cells is vital.
Current fluorescence-activated cell sorting (FACS) methods are limited to cell surface markers and require cells to be alive.

Purpose of the Study:

To develop a universal method for sorting cells based on intracellular antigens.
To enable gene expression analysis of sorted cells without RNA degradation.

Main Methods:

Established a protocol for messenger RNA quantification after FACS (FACS-mQ).
Targeted intracellular antigens for cell sorting.

Main Results:

Successfully developed FACS-mQ protocol for intracellular antigen-based cell sorting.

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Last Updated: Jun 12, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Enabled RNA quantification from sorted cells, preserving gene expression profiles.

Conclusions:

FACS-mQ offers a simpler, universal approach for analyzing cells with specific gene expression patterns.
This method is applicable for detecting and analyzing stem cells and cancer stem cells in various tissues.