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Updated: Jun 12, 2026

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
In vivo dentin microhardness beneath a calcium-phosphate cement
E Bresciani1, W C Wagner, M F L Navarro
1University of Michigan, Department of Cariology, Restorative Sciences and Endodontics, Ann Arbor, MI, USA.
Mineral-releasing calcium-phosphate cement (Ca-PO(4)) aids in remineralizing dentin. Acid etching before applying the cement significantly boosted dentin microhardness after 3 months in vivo.
Area of Science:
- Dentistry
- Biomaterials Science
- Cariology
Background:
- Minimally invasive caries removal preserves dentin.
- Mineral-releasing cements can promote dentin remineralization.
- Indirect pulp capping aims to maintain pulp vitality.
Purpose of the Study:
- Evaluate the in vivo remineralization of caries-affected dentin using resin-based calcium-phosphate cement (Ca-PO(4)).
- Assess the effect of acid etching on the remineralization capacity of the cement.
Main Methods:
- Permanent teeth indicated for extraction were excavated and restored with or without Ca-PO(4) base.
- Teeth were restored with adhesive restorations.
- After 3 months in vivo, teeth were extracted for in vitro microhardness analysis of the cavity floor.
Main Results:
- Caries-affected dentin treated with acid etching prior to Ca-PO(4) cement showed significantly increased Knoop microhardness.
- The non-etched Ca-PO(4) group showed similar results to the control (no cement).
- Acid etching enhanced the microhardness of residual dentin near the interface.
Conclusions:
- Acid etching prior to Ca-PO(4) cement application enhances dentin remineralization and microhardness.
- This technique shows promise for preserving and repairing caries-affected dentin.
- Resin-based Ca-PO(4) cement is a viable option for indirect pulp capping when combined with appropriate surface treatment.
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