Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remote homology and functional genetics unmask deeply preserved Scm3/HJURP orthologs in metazoans.

Science advances·2026
Same author

Integrated multi-technology exploration of the mechanism by which Badushengji San regulates core targets in diabetic foot ulcer.

Molecular genetics and genomics : MGG·2026
Same author

A second life.

Genetics·2026
Same author

Ultrasound-Guided Drug Injection Combined With Suprascapular Nerve Pulsed Radiofrequency for Post-Stroke Hemiplegic Shoulder Pain: A Randomized Controlled Trial.

American journal of physical medicine & rehabilitation·2026
Same author

Synaptic and neural pathway redundancy enables the robustness of a sensory-motor reflex and promotes predation escape in <i>Caenorhabditis elegans</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-performance quarter-wave plate based on the effective graded impedance matching configuration at 220 GHz.

Optics express·2026

Related Experiment Video

Updated: Jun 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

Enhanced neuronal RNAi in C. elegans using SID-1.

Andrea Calixto1, Dattananda Chelur, Irini Topalidou

  • 1Department of Biological Sciences, Columbia University, New York, New York, USA.

Nature Methods
|June 1, 2010
PubMed
Summary
This summary is machine-generated.

We enhanced systemic RNA interference (RNAi) in C. elegans neurons by expressing the SID-1 protein. This technique allows for the study of neuronal gene function, including lethal genes, and identified roles for integrin signaling in mechanosensation.

More Related Videos

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
09:06

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Published on: September 18, 2020

Related Experiment Videos

Last Updated: Jun 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
09:06

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Published on: September 18, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • Systemic RNA interference (RNAi) is a powerful tool for gene silencing in C. elegans.
  • The SID-1 transmembrane protein mediates dsRNA uptake for systemic RNAi.
  • Targeting RNAi specifically to neurons is crucial for studying neuronal gene function.

Purpose of the Study:

  • To investigate the role of neuronal SID-1 expression in enhancing systemic RNAi.
  • To develop a method for studying neuronal gene function, including essential genes, using RNAi.
  • To identify novel genes involved in neuronal processes, such as mechanosensation.

Main Methods:

  • Expressing the SID-1 gene in C. elegans neurons.
  • Utilizing feeding RNAi with dsRNA targeting neuronal genes.
  • Employing mutations in lin-15b and lin-35 to enhance RNAi effects.
  • Generating transgenic C. elegans strains with cell-specific SID-1 expression.

Main Results:

  • Neuronal SID-1 expression significantly enhanced the RNAi response in C. elegans neurons.
  • RNAi phenotypes were observed for both known and uncharacterized neuronal genes.
  • Neuronal SID-1 expression acted as a dsRNA sink, reducing non-neuronal RNAi.
  • Cell-specific expression of SID-1 enabled targeted RNAi studies.
  • A role for integrin signaling genes in mechanosensation was identified.

Conclusions:

  • Neuronal expression of SID-1 is an effective strategy to enhance systemic RNAi in C. elegans.
  • This approach facilitates the study of neuronal gene function, including genes essential for viability.
  • The developed system can uncover novel gene functions in specific cell types, exemplified by the identification of integrin signaling in mechanosensation.