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Updated: Jun 12, 2026

Mouse Models Of Helicobacter Infection And Gastric Pathologies
07:43

Mouse Models Of Helicobacter Infection And Gastric Pathologies

Published on: October 18, 2018

Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated

Cinghu Senthilkumar1, Sivasithambaram Niranjali, Venkatraman Jayanthi

  • 1Unit of Biochemistry, Department of Zoology, School of Life Sciences, University of Madras, Guindy Campus, Chennai, 600 025 Tamil Nadu, India.

Journal of Cancer Research and Clinical Oncology
|June 1, 2010
PubMed
Abstract

Insights

Helicobacter pylori (H. pylori) infection significantly increases tumor necrosis factor-alpha (TNF-α) in the stomach. This elevated TNF-α expression correlates with increased risk from preneoplastic lesions to gastric cancer.

Area of Science:

  • Gastroenterology
  • Oncology
  • Immunology

Background:

  • Helicobacter pylori (H. pylori) infection is linked to gastric mucosal damage.
  • H. pylori stimulates inflammatory cytokine production, including tumor necrosis factor-alpha (TNF-α).
  • In vivo inflammatory responses in gastric mucosa during H. pylori infection require further investigation.

Purpose of the Study:

  • To investigate TNF-α expression induced by H. pylori infection.
  • To understand TNF-α's role in gastric carcinogenesis initiation and promotion.

Main Methods:

  • Analysis of 200 patients with varying gastric mucosal conditions (normal, gastritis, metaplasia, dysplasia, adenocarcinoma).
  • Quantification of TNF-α mRNA and protein levels using RT-PCR and Western blotting.
  • Semiquantitative assessment of TNF-α localization via immunohistochemistry.

Main Results:

  • Significantly increased TNF-α mRNA and protein expression observed in chronic gastritis, intestinal metaplasia, dysplasia, and gastric adenocarcinoma.
  • Immunohistochemistry confirmed elevated TNF-α expression in these conditions.

Conclusions:

  • Overexpression of TNF-α demonstrates a significant severity-dose-response relationship.
  • TNF-α serves as a risk marker progressing from preneoplastic lesions to gastric cancer.

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