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Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates
Joske Ruytinx1, Adrian R Craciun, Karen Verstraelen
1Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, 3590 Diepenbeek, Belgium.
Mycorrhiza
|June 1, 2010
Summary
Suillus luteus, a pioneer fungus, shows significant gene expression variation. This adaptability may help the ectomycorrhizal symbiont colonize diverse environments, including heavy metal-contaminated soils.
Area of Science:
- Mycology
- Environmental Science
- Genomics
Background:
- Suillus luteus (L.:Fr.) is an ectomycorrhizal basidiomycete pioneer species.
- It commonly associates with young pines on disturbed and heavy metal-contaminated sites.
- Intraspecific variation in S. luteus may facilitate adaptation to novel environments.
Purpose of the Study:
- To investigate transcriptomic differences in cadmium (Cd)-sensitive and Cd-tolerant Suillus luteus isolates.
- To identify differentially expressed genes related to heavy metal tolerance.
- To discuss the potential roles of identified proteins in Cd accumulation and tolerance.
Main Methods:
- Transcriptomic analysis using complementary DNA-amplified fragment length polymorphism (cDNA-AFLP).
- Comparison of gene expression profiles between Cd-sensitive and Cd-tolerant S. luteus isolates.
- Isolation of S. luteus from non-polluted and heavy metal-polluted sites in Limburg, Belgium.
Main Results:
- Significant intraspecific variation in transcript profiles was observed in Suillus luteus.
- This variation was comparable between Cd-sensitive and Cd-tolerant isolates.
- Differentially expressed genes were identified, suggesting roles in heavy metal tolerance.
Conclusions:
- Suillus luteus exhibits substantial transcriptomic plasticity.
- This variation is present in both Cd-sensitive and Cd-tolerant populations.
- Such adaptability is crucial for the pioneer species' colonization of challenging environments, including those with heavy metal contamination.
