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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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Solid-phase screening of monoclonal antibodies
1Department of Experimental Immunobiology, Wellcome Research Laboratories, Beckenham, Kent.
By far the most convenient and simple assays for screening monoclonal antibody-producing hybridomas are those utilizing solid-phase binding assays, where radiolabelled or enzyme linked antispecies antibodies are used to detect binding of the hybridoma supernatants to insolubilized antigen. Such procedures require an adequate supply of antigen, preferably purified, which can be bound to a solid support, usually through noncovalent binding to PVC microtiter plates or nitrocellulose sheets.
By far the most convenient and simple assays for screening monoclonal antibody-producing hybridomas are those utilizing solid-phase binding assays, where radiolabelled or enzyme linked antispecies antibodies are used to detect binding of the hybridoma supernatants to insolubilized antigen. Such procedures require an adequate supply of antigen, preferably purified, which can be bound to a solid support, usually through noncovalent binding to PVC microtiter plates or nitrocellulose sheets.
