Related Experiment Video
Updated: Jun 12, 2026

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
Published on: December 3, 2014
Rocket immunoelectrophoresis
1School of Biological and Environmental Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, England.
Abstract:
Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein mixture. The method, originally introduced by Laurell (1) involves a comparison of the sample of unknown concentration with a series of dilutions of a known concentration of the protein, and requires a monospecific antiserum against the protein under investigation. The samples to be compared are loaded side-by-side in small circular wells along the edge of an agarose gel that contains the monospecific antibody. These samples (antigen) are then electrophoresed into the agarose gel where interaction between antigen and antibody takes place. In the presence of excess antigen, the antigen#x2013;antibody complex is soluble, but as the antigen moves further into the gel, more antigen combines with antibody until a point of equivalence is reached. At this stage the antigen#x2013;antibody complex is insoluble. The end result is a precipitation 'rocket' spreading out from the loading well.
More Related Videos
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Electrophoresis: Overview
There...
Immunogold Electron Microscopy

