Related Experiment Video
Updated: Jun 12, 2026
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Published on: September 1, 2015
Increased PD-L1 expression and PD-L1/CD86 ratio on dendritic cells were associated with impaired dendritic cells
Tao Shen1, Xiangmei Chen, Yu Chen
1Department of Microbiology, Peking University Health Science Center, Beijing, China. taoshen@hsc.pku.edu.cn
Abstract:
Impaired hepatitis C virus (HCV)-specific T cell immunity was associated with the persistence of HCV infection. Dysfunction of dentritic cells (DCs) was believed to be involved in T cell exhaustion, but the mechanisms were rarely understood. In this study, surface costimulatory marker (CD83, CD86, and CD40), coinhibitory marker (PD-L1) expression and allostimulatory capacity of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were evaluated in HCV-infected patients. Results showed that the expression of both costimulatory and coinhibitory markers was increased in HCV-infected patients compared with healthy controls. PD-L1/CD86 ratio was increased and positively correlated with PD-L1 expression on DCs in HCV-infected patients. Allostimulatory capacity of DCs was impaired and inversely correlated with PD-L1 expression and PD-L1/CD86 ratio. These findings suggested that the effect of inhibitory marker PD-L1 overwhelmed the effect of costimulatory markers and down regulated DC-T activation in HCV-infected patients. The results will be helpful to understand the mechanism of dysfunction of DCs in HCV infection and shed light on the DC-based immunotherapeutic strategy.
