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Correlation between rotavirus A genotypes detected in hospitalized children and sewage samples in 2006, Córdoba,

P A Barril1, M O Giordano, M B Isa

  • 1Virology Institute Dr. J M Vanella, School of Medical Sciences, National University of Cordoba, Córdoba, Argentina. patricia_barril@yahoo.com.ar

Journal of Medical Virology
|June 1, 2010
PubMed

Insights

Sewage surveillance effectively tracks rotavirus A (RV-A) genotypes, mirroring clinical data and revealing year-round circulation. This method offers a broader population view than relying solely on symptomatic cases.

Area of Science:

  • Environmental microbiology
  • Virology
  • Public health surveillance

Background:

  • Routine rotavirus A (RV-A) surveillance relies on clinical cases, potentially underrepresenting the full spectrum of circulating strains.
  • Understanding RV-A genotype distribution and seasonality is crucial for effective public health interventions.

Purpose of the Study:

  • To assess if sewage surveillance accurately reflects RV-A genotypes and seasonal patterns observed in clinical diarrheal cases.
  • To determine the utility of wastewater-based epidemiology for comprehensive RV-A monitoring.

Main Methods:

  • Comparison of RV-A VP7 gene genotypes from 52 sewage effluent samples and 70 clinical stool specimens collected over 12 months (2006).
  • Concentration of sewage samples and RNA extraction for subsequent genotyping.
  • Proportional distribution analysis of detected RV-A G-genotypes between sewage and clinical datasets.

Main Results:

  • Similar proportional distributions of major RV-A G-genotypes (G1, G3, G2, G4, G9) were observed in both sewage and clinical samples during the cold season.
  • Sewage surveillance indicated consistent RV-A circulation throughout the year, contrasting with the seasonal peak in community infections.
  • A significant correlation was found between RV-A genotypes detected in sewage and those from diarrheal patients.

Conclusions:

  • Wastewater surveillance is a viable and representative method for monitoring RV-A genotypes in a population.
  • Sewage monitoring provides a more complete picture of year-round RV-A circulation than traditional clinical surveillance.
  • This approach can enhance public health strategies by offering continuous insights into viral epidemiology.

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